Alterocin, an Antibiofilm Protein Secreted by Pseudoalteromonas sp. Strain 3J6.

ABSTRACT: We sought to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. strain 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced, and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another nonactive Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein that includes a predicted

SUBMITTER: Jouault A 

PROVIDER: S-EPMC7531970 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature