SequelTools: a suite of tools for working with PacBio Sequel raw sequence data.

ABSTRACT:

Background

PacBio sequencing is an incredibly valuable third-generation DNA sequencing method due to very long read lengths, ability to detect methylated bases, and its real-time sequencing methodology. Yet, hitherto no tool was available for analyzing the quality of, subsampling, and filtering PacBio data.

Results

Here we present SequelTools, a command-line program containing three tools: Quality Control, Read Subsampling, and Read Filtering. The Quality Control tool quickly processes PacBio Sequel raw sequence data from multiple SMRTcells producing multiple statistics and publication-quality plots describing the quality of the data including N50, read length and count statistics, PSR, and ZOR. The Read Subsampling tool allows the user to subsample reads by one or more of t

SUBMITTER: Hufnagel DE 

PROVIDER: S-EPMC7532105 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature