Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury.

ABSTRACT: Background: X-inactive specific transcript (Xist) is a lncRNA, which plays a significant role in X-chromosome inactivation, regulates cell proliferation in tumor cells, and inhibits apoptosis in acute myocardial infarction. On the other hand, miR-7a-5p is involved in cardiomyocytes injury in myocardial ischemia/reperfusion. However, their roles in LPS-induced damage remain unclear. Objectives: This study aimed at using siRNA transfection and lentivirus infection to regulate the expression of xist and miR-7a-5p, and to evaluate their effects on LPS-induced myocardial damage. Method: Mice cardiomyocytes (MCM) cells were divided into six groups, namely the control group, the LPS group, the LPS + lncRNA^- group, the LPS + lncRNA⁺ group, the LPS + miRNA

SUBMITTER: Liang D 

PROVIDER: S-EPMC7532474 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature