A smartphone-based particle diffusometry platform for sub-attomolar detection of Vibrio cholerae in environmental water.

ABSTRACT: Each year, 3.4 million people die from waterborne diseases worldwide. Development of a rapid and portable platform for detecting and monitoring waterborne pathogens would significantly aid in reducing the incidence and spread of infectious diseases. By combining optical methods and smartphone technology with molecular assays, the sensitivity required to detect exceedingly low concentrations of waterborne pathogens can readily be achieved. Here, we implement smartphone-based particle diffusometry (PD) detection of loop-mediated isothermal amplification (LAMP) targeting the waterborne pathogen Vibrio cholerae (V. cholerae). By measuring the diffusion of 400 nm streptavidin-coated fluorescent nanoparticles imaged at 68X magnification on a smartphone, we can detect as few as 6 V. cholerae cell

SUBMITTER: Moehling TJ 

PROVIDER: S-EPMC7532658 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature