PlpE Epitopes of Pasteurella multocida Fusion Protein as Novel Subunit Vaccine Candidates.

ABSTRACT:

Background

Pasteurella multocida is the causative agent of many diseases. Antimicrobial treatment disadvantages highlight the need to find other possible ways such as prophylaxis to manage infections. Current vaccines against this agent include inactivated bacteria, live-attenuated bacteria, and nonpathogenic bacteria, which have disadvantages such as lack of immunogenicity, reactogenicity, or reversion to virulence wild bacteria. Using bioinformatical approaches, potentially immunogenic and protective epitopes identified and merged to design the best epitope fusion form in case of immunogenicity as a vaccine candidate.

Materials and methods

In this study, the fusion protein (PlpE1 + 2 + 3) and full PlpE genes (PlpE-Total) were cloned in pET28a in

SUBMITTER: Mostaan S 

PROVIDER: S-EPMC7532835 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature