Automated lipid droplet quantification system for phenotypic analysis of adipocytes using CellProfiler.

ABSTRACT: Adipogenic differentiation is the process by which preadipocytes become mature adipocytes, cells that store energy and regulate metabolic homeostasis. During differentiation, neutral lipids that accumulate in adipocytes can be detected using stains and used as an index of cell differentiation. However, imaging tools for evaluating intracellular lipid droplets remain at their infancy. Nutrition, stress, or chemical exposure can dysregulate adipogenic differentiation and lipid metabolism. Therefore, the aims of this study were to develop an accurate, standardized approach to quantify lipid droplet size of mature adipocytes and a clustering approach to analyze the total lipid content per adipocyte. For the lipid droplet analysis, we used two approaches, the free online computer software of re

SUBMITTER: Adomshick V 

PROVIDER: S-EPMC7533149 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature