A Novel bla _CTX-M-65-Harboring IncHI2 Plasmid pE648CTX-M-65 Isolated from a Clinical Extensively-Drug-Resistant Escherichia coli ST648.

ABSTRACT:

Background

An ESBL, carbapenemase- and MCR-1-producing Escherichia coli ST648 strain was isolated from the urine sample of a patient in a Chinese tertiary hospital in 2016.

Methods

The strain was fully sequenced by GridION X5 platform of Oxford Nanopore Technology.

Results

The sequence analysis showed that the extended-spectrum β-lactamases CTX-M-65 and OXA-1, the carbapenemase NDM-5, the MCR-1 were encoded, respectively, by three different resistance plasmids. The pE648CTX-M-65-carrying bla _CTX-M-65 was a novel conjugative plasmid belonging to IncHI2 type; except for the bla _CTX-M-65, it also carried resistance genes ble, floR, sul1, aph(4)-Ia, aac(3)-VI, aac(6')-II, bla _OXA-1, catB, arr3 and tetA

SUBMITTER: Lu Y 

PROVIDER: S-EPMC7533269 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature