Ribulose 1,5-bisphosphate carboxylase/oxygenase activates O₂ by electron transfer.

ABSTRACT: Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the cornerstone of atmospheric CO₂ fixation by the biosphere. It catalyzes the addition of CO₂ onto enolized ribulose 1,5-bisphosphate (RuBP), producing 3-phosphoglycerate which is then converted to sugars. The major problem of this reaction is competitive O₂ addition, which forms a phosphorylated product (2-phosphoglycolate) that must be recycled by a series of biochemical reactions (photorespiratory metabolism). However, the way the enzyme activates O₂ is still unknown. Here, we used isotope effects (with ²H, ²⁵Mg, and ¹⁸O) to monitor O₂ activation and assess the influence of outer sphere atoms, in two Rubisco forms of contrasted O₂

SUBMITTER: Bathellier C 

PROVIDER: S-EPMC7533879 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature