Inherent Immune Cell Variation Within Colonic Segments Presents Challenges for Clinical Trial Design.

ABSTRACT:

Background and aims

Intestinal biopsy sampling during IBD trials represents a valuable adjunct strategy for understanding drug responses at the tissue level. Given the length and distinctive embryonic origins of the proximal and distal colon, we investigated whether inherent regional differences of immune cell composition could introduce confounders when sampling different disease stages, or pre/post drug administration. Here, we capitalise on novel mass cytometry technology to perform deep immunophenotyping of distinct healthy colonic segments, using the limited numbers of biopsies that can be harvested from patients.

Methods

Biopsies [2.8 mm] were collected from the caecum, transverse colon, descending colon, and rectum of normal volunteers. Intestinal leukocytes were isol

SUBMITTER: Tyler CJ 

PROVIDER: S-EPMC7533898 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature