Sulphonamide inhibition profile of Staphylococcus aureus β-carbonic anhydrase.

ABSTRACT: This paper presents the production and kinetic and inhibitory characterisation of β-carbonic anhydrase from the opportunistic bacterium Staphylococcus aureus (SauBCA). From the eight different carbonic anhydrase (CA) families known to date, humans have only the α-form, whereas many clinically relevant pathogens have β- and/or γ-form(s). Based on this discovery, β- and γ-CAs have been introduced as promising new anti-infective targets. The results of this study revealed that recombinant SauBCA possesses significant CO₂ hydration activity with a k_cat of 1.46 × 10⁵ s^-1 and a k_cat/K_M of 2.56 × 10⁷ s^{- 1}M^-1. Its enzymatic function was inhibited by various sulphonamides in the n

SUBMITTER: Urbanski LJ 

PROVIDER: S-EPMC7534311 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature