"Mind the Gap": Hi-C Technology Boosts Contiguity of the Globe Artichoke Genome in Low-Recombination Regions.

ABSTRACT: Globe artichoke (Cynara cardunculus var. scolymus; 2n2x=34) is cropped largely in the Mediterranean region, being Italy the leading world producer; however, over time, its cultivation has spread to the Americas and China. In 2016, we released the first (v1.0) globe artichoke genome sequence (http://www.artichokegenome.unito.it/). Its assembly was generated using ∼133-fold Illumina sequencing data, covering 725 of the 1,084 Mb genome, of which 526 Mb (73%) were anchored to 17 chromosomal pseudomolecules. Based on v1.0 sequencing data, we generated a new genome assembly (v2.0), obtained from a Hi-C (Dovetail) genomic library, and which improves the scaffold N₅₀ from 126 kb to 44.8 Mb (∼356-fold increase) and N₉₀ from 29 kb to 17.8 Mb (∼685-fold increase).

SUBMITTER: Acquadro A 

PROVIDER: S-EPMC7534446 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature