Identification of Essential Genes and Fluconazole Susceptibility Genes in Candida glabrata by Profiling Hermes Transposon Insertions.

ABSTRACT: Within the budding yeasts, the opportunistic pathogen Candida glabrata and other members of the Nakaseomyces clade have developed virulence traits independently from C . albicans and C. auris To begin exploring the genetic basis of C. glabrata virulence and its innate resistance to antifungals, we launched the Hermes transposon from a plasmid and sequenced more than 500,000 different semi-random insertions throughout the genome. With machine learning, we identified 1278 protein-encoding genes (25% of total) that could not tolerate transposon insertions and are likely essential for C. glabrata fitness in vitro Interestingly, genes involved in mRNA splicing were less likely to be essential in C. glabrata than their ortholo

SUBMITTER: Gale AN 

PROVIDER: S-EPMC7534453 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature