In Vivo NADH/NAD⁺ Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants.

ABSTRACT: NADH and NAD⁺ are a ubiquitous cellular redox couple. Although the central role of NAD in plant metabolism and its regulatory role have been investigated extensively at the biochemical level, analyzing the subcellular redox dynamics of NAD in living plant tissues has been challenging. Here, we established live monitoring of NADH/NAD⁺ in plants using the genetically encoded fluorescent biosensor Peredox-mCherry. We established Peredox-mCherry lines of Arabidopsis (Arabidopsis thaliana) and validated the biophysical and biochemical properties of the sensor that are critical for in planta measurements, including specificity, pH stability, and reversibility. We generated an NAD redox atlas of the cytosol of living Arabidopsis seedlings that revealed pronounced diff

SUBMITTER: Steinbeck J 

PROVIDER: S-EPMC7534465 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature