Engineering anisotropic 3D tubular tissues with flexible thermoresponsive nanofabricated substrates.

ABSTRACT: Tissue engineering aims to capture the structural and functional aspects of diverse tissue types in vitro. However, most approaches are limited in their ability to produce complex 3D geometries that are essential for tissue function. Tissues, such as the vasculature or chambers of the heart, often possess curved surfaces and hollow lumens that are difficult to recapitulate given their anisotropic architecture. Cell-sheet engineering techniques using thermoresponsive substrates provide a means to stack individual layers of cells with spatial control to create dense, scaffold-free tissues. In this study, we developed a novel method to fabricate complex 3D structures by layering multiple sheets of aligned cells onto flexible scaffolds and casting them into hollow tubular geometries using cust

SUBMITTER: Williams NP 

PROVIDER: S-EPMC7536133 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature