Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads.

ABSTRACT: Psychrotrophic Pseudomonas species are the key spoilage bacteria of aerobically stored chilled meat. These organisms readily form biofilms on meat under refrigerated conditions leading to consumer rejection and associated economic losses. Limited information is available on the matrix composition of the biofilms formed by these bacteria. We quantified and characterized the main components of the matrix of mono-species biofilms of selected Pseudomonas fragi and Pseudomonas lundensis strains using chemical analysis and Raman spectroscopy. The biofilms were grown at 10 °C and 25 °C on nitro-cellulose membranes placed on surface sterilized beef cuts. Extra-cellular polymeric substances of the matrix were extracted in soluble and bound forms and were chemically assessed for total carbohydrates,

SUBMITTER: Wickramasinghe NN 

PROVIDER: S-EPMC7536239 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature