Long Non-Coding RNA H19 Participates in Periodontal Inflammation via Activation of Autophagy.

ABSTRACT:

Purpose

Periodontitis is the leading cause of tooth loss. The role of long non-coding RNA (lncRNA) in periodontal inflammation remains unclear. The aim of this study was to investigate the role of lncRNA H19 in periodontitis and its possible regulation of autophagy in periodontitis.

Material and methods

Inflammation level was determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) in periodontal ligament cells (PDLCs). Western blotting, flow cytometric analysis, and immunofluorescence staining were used to detect the autophagy flux. Overexpression or knockdown of H19 was used to confirm its function. Ligature-induced periodontitis model in mice and periodontitis-affected human gingival tissue were use

SUBMITTER: Guo R 

PROVIDER: S-EPMC7536258 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature