Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous Fungus Aspergillus oryzae.

ABSTRACT: The machinery for mRNA localization is one of crucial molecular structures allowing cellular spatiotemporal organization of protein synthesis. Although the molecular mechanisms underlying mRNA localization have been thoroughly investigated in unicellular organisms, little is known about multicellular and multinuclear filamentous fungi. Here, we conducted single-molecule fluorescence in situ hybridization (smFISH) to first visualize the mRNA molecules of α-amylase, which are encoded by amyB, and which are thought to be abundantly secreted from the hyphal tips of the industrially important fungus Aspergillus oryzae. Consistent with previous biochemical studies, fluorescein amidite (FAM) fluorescence derived from amyB expression was observed in A. oryzae hyp

SUBMITTER: Higuchi Y 

PROVIDER: S-EPMC7536267 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature