Development of PMAxx^TM-Based qPCR for the Quantification of Viable and Non-viable Load of Salmonella From Poultry Environment.

ABSTRACT: Determining the viable and non-viable load of foodborne pathogens in animal production can be useful in reducing the number of human outbreaks. In this study, we optimized a PMAxx^TM-based qPCR for quantifying viable and non-viable load of Salmonella from soil collected from free range poultry environment. The optimized nucleic acid extraction method resulted in a significantly higher (P < 0.05) yield and quality of DNA from the pure culture and Salmonella inoculated soil samples. The optimized primer for the amplification of the invA gene fragment showed high target specificity and a minimum detection limit of 10² viable Salmonella from soil samples. To test the optimized PMAxx^TM-based qPCR assay, soil obtained from a free

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7536286 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature