Establishment of a Real-Time Recombinase Polymerase Amplification Assay for the Detection of Avian Reovirus.

ABSTRACT: Avian reovirus (ARV) infection results in multiple disease manifestations in chicken. A rapid detection method will contribute to early diagnosis and control of the virus infection. The recombinase polymerase amplification (RPA) technology is a nucleic acid amplification method which is experiencing rapid development. In present study, a real-time reverse transcription (RT)-RPA assay was developed for the detection of ARV. The limit of detection of the real-time RT-RPA was 10² copies/μL of ARV genomic RNA standard in 95% of cases. The RT-RPA assay also exhibited remarkable specificity. When the nucleic acids of CRV and other common avian pathogens were subjected to the RT-RPA test, only ARV tested positive, all the other pathogens tested negative. Furthermore, the practicality o

SUBMITTER: Ma L 

PROVIDER: S-EPMC7536300 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature