Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples.
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ABSTRACT:
SUBMITTER: Wagner JT
PROVIDER: S-EPMC7536441 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
Diurnal stability of cell-free DNA and cell-free RNA in human plasma samples.
Wagner Josiah T JT Kim Hyun Ji HJ Johnson-Camacho Katie C KC Kelley Taylor T Newell Laura F LF Spellman Paul T PT Ngo Thuy T M TTM
Scientific reports 20201005 1
Many emerging technologies are reliant on circulating cell-free DNA (cfDNA) and cell-free RNA (cfRNA) applications in the clinic. However, the impact of diurnal cycles or daily meals on circulating analytes are poorly understood and may be confounding factors when developing diagnostic platforms. To begin addressing this knowledge gap, we obtained plasma from four healthy donors serially sampled five times during 12 h in a single day. For all samples, we measured concentrations of cfDNA and cfRN ...[more]
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