Comparison of the diagnostic efficacy between two PCR test kits for SARS-CoV-2 nucleic acid detection.

ABSTRACT:

Background

To compare the diagnostic efficacy between two different real-time reverse transcription polymerase chain reaction (RT-PCR) test kits for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection and provide references for laboratories.

Methods

Throat swab samples from 18 hospitalized patients were clinically diagnosed with coronavirus disease 2019 (COVID-19) and 100 hospitalized patients without COVID-19 were collected. SARS-CoV-2 nucleic acid was detected in throat swab samples with RT-PCR test kits from Sansure Biotech Inc (Hunan, China) and Shanghai BioGerm Medical Biotechnology Co., Ltd.(Shanghai, China). The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and kappa value were analyzed, and

SUBMITTER: Lu Y 

PROVIDER: S-EPMC7536918 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature