Accuracy of serological testing for SARS-CoV-2 antibodies: First results of a large mixed-method evaluation study.

ABSTRACT:

Background

Serological immunoassays that can identify protective immunity against SARS-CoV-2 are needed to adapt quarantine measures, assess vaccination responses, and evaluate donor plasma. To date, however, the utility of such immunoassays remains unclear. In a mixed-design evaluation study, we compared the diagnostic accuracy of serological immunoassays that are based on various SARS-CoV-2 proteins and assessed the neutralizing activity of antibodies in patient sera.

Methods

Consecutive patients admitted with confirmed SARS-CoV-2 infection were prospectively followed alongside medical staff and biobank samples from winter 2018/2019. An in-house enzyme-linked immunosorbent assay utilizing recombinant receptor-binding domain (RBD) of the SARS-CoV-2 spike protein was develop

SUBMITTER: Brigger D 

PROVIDER: S-EPMC7537154 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature