A Novel Butanol Tolerance-Promoting Function of the Transcription Factor Rob in Escherichia coli.

ABSTRACT: Producing high concentrations of biobutanol is challenging, primarily because of the toxicity of butanol toward cells. In our previous study, several butanol tolerance-promoting genes were identified from butanol-tolerant Escherichia coli mutants and inactivation of the transcriptional regulator factor Rob was shown to improve butanol tolerance. Here, the butanol tolerance characteristics and mechanism regulated by inactivated Rob are investigated. Comparative transcriptome analysis of strain DTrob, with a truncated rob in the genome, and the control BW25113 revealed 285 differentially expressed genes (DEGs) to be associated with butanol tolerance and categorized as having transport, localization, and oxidoreductase activities. Expression of 25 DEGs representing differ

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7537768 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature