Host-induced spermidine production in motile Pseudomonas aeruginosa triggers phagocytic uptake.

ABSTRACT: Exploring the complexity of host-pathogen communication is vital to understand why microbes persist within a host, while others are cleared. Here, we employed a dual-sequencing approach to unravel conversational turn-taking of dynamic host-pathogen communications. We demonstrate that upon hitting a host cell, motile Pseudomonas aeruginosa induce a specific gene expression program. This results in the expression of spermidine on the surface, which specifically activates the PIP₃-pathway to induce phagocytic uptake into primary or immortalized murine cells. Non-motile bacteria are more immunogenic due to a lower expression of arnT upon host-cell contact, but do not produce spermidine and are phagocytosed less. We demonstrate that not only the presence of pathogen inh

SUBMITTER: Felgner S 

PROVIDER: S-EPMC7538158 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature