Millisecond dynamics of an unlabeled amino acid transporter.

ABSTRACT: Excitatory amino acid transporters (EAATs) are important in many physiological processes and crucial for the removal of excitatory amino acids from the synaptic cleft. Here, we develop and apply high-speed atomic force microscopy line-scanning (HS-AFM-LS) combined with automated state assignment and transition analysis for the determination of transport dynamics of unlabeled membrane-reconstituted Glt_Ph, a prokaryotic EAAT homologue, with millisecond temporal resolution. We find that Glt_Ph transporters can operate much faster than previously reported, with state dwell-times in the 50 ms range, and report the kinetics of an intermediate transport state with height between the outward- and inward-facing states. Transport domains stochastically probe transmembrane motion

SUBMITTER: Matin TR 

PROVIDER: S-EPMC7538599 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature