Rapid Determination of Antimicrobial Susceptibility by Stimulated Raman Scattering Imaging of D₂O Metabolic Incorporation in a Single Bacterium.

ABSTRACT: Rapid antimicrobial susceptibility testing (AST) is urgently needed for treating infections with appropriate antibiotics and slowing down the emergence of antibiotic-resistant bacteria. Here, a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging of deuterium oxide (D₂O) metabolism is reported. Metabolic incorporation of D₂O into biomass in a single bacterium and the metabolic response to antibiotics are probed in as short as 10 min after culture in 70% D₂O medium, the fastest among current technologies. Single-cell metabolism inactivation concentration (SC-MIC) is obtained in less than 2.5 h from colony to results. The SC-MIC results of 37 sets of bacterial isolate samples, which include 8 major bacterial

SUBMITTER: Zhang M 

PROVIDER: S-EPMC7539191 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature