TRADES: Targeted RNA Demethylation by SunTag System.

ABSTRACT: N6-methyladenosine (m⁶A) is rapidly being studied and uncovered to play a significant role in various biological processes as well as in RNA fate and functions, while the effects of defined m⁶A sites are rarely characterized for the lack of convenient tools. To provide an applicable method to remove m⁶A modification at specific loci, an m⁶A editing system called "targeted RNA demethylation by SunTag system (TRADES)" is engineered. In this system, the targeting element dCas13b is fused to ten copies of GCN4 peptides which can recruit multiple scFv-fusion RNA demethylase to demethylate the target m⁶A site. Owing to this design, TRADES is more flexible to the indistinct m⁶A sites for its wide editing window. By site-specific de

SUBMITTER: Mo J 

PROVIDER: S-EPMC7539198 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature