Dataset Information

Optimization of Cr⁶⁺ Removal by Bacillus subtilis Strain SZMC 6179J from Chromium-Containing Soil.

ABSTRACT: The removal rate of Cr⁶⁺ has been explored by the optimized removal conditions. Five Cr-resistant strains were isolated from chromium-contained soil. The most efficient strain S1 was identified as Bacillus subtilis strain SZMC 6179J through 16S rDNA. Response surface methodology (RSM) was used to investigate the effects of four independent variables, including initial pH, initial Cr⁶⁺ concentration (mg/L), time (h) and inoculation percentage (%). RSM revealed that when pH was 5.02, time was 24.0 h, inoculation percentage was 4.64% (v/v) and initial concentration of Cr⁶⁺ was 55.0 mg/L, the optimal condition was obtained. Under the optimum conditions, the actual response values for Bacillus subtilis strain SZMC 6179J was 93.50%. The pH was the most significant factor towards removal rate of Cr⁶⁺. The result showed that the removal mechanism of Cr⁶⁺ by Bacillus subtilis strain SZMC 6179J was reduction under normal conditions. The removal mechanism of Cr⁶⁺ by Bacillus subtilis strain SZMC 6179J was adsorption under adverse conditions.

SUBMITTER: Liu J

PROVIDER: S-EPMC7539242 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Optimization of Cr6+ Removal by Bacillus subtilis Strain SZMC 6179J from Chromium-Containing Soil.

Liu Jianghong J Xue Jian J Wei Xiaohang X Su Huimin H Xu Ruidan R

Indian journal of microbiology 20200523 4

The removal rate of Cr6+ has been explored by the optimized removal conditions. Five Cr-resistant strains were isolated from chromium-contained soil. The most efficient strain S1 was identified as Bacillus subtilis strain SZMC 6179J through 16S rDNA. Response surface methodology (RSM) was used to investigate the effects of four independent variables, including initial pH, initial Cr6+ concentration (mg/L), time (h) and inoculation percentage (%). RSM revealed that wh ...[more]

PMID: 33087992

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Project description:Valorization of poultry waste is a significant challenge addressed in this study, which aimed to produce cost-effective and sustainable peptones from poultry waste. The isolation process yielded the highly potent proteolytic B.subtilis isolate P6, identified through 16S rRNA gene sequencing to share 94% similarity with the B.subtilis strain KEMET024 (GenBank accession number PP694485.1) and deposited in MIRCEN culture collection, Cairo, Egypt as EMCC 998871. It reached optimal production levels during 24 h of incubation, with biomass at 2.5 g/L, protease activity at 455 U/mL, and total amino acid (TAA) concentration at 208 mg/mL. For screening the most significant factors for peptone production, the Plackett-Burman design identified meat and bone meal concentration as the main significant factor influencing total amino acid reaching 420 mg/mL. BOX-Behnken design optimized peptone production increasing its production level by twofold to reach 2850 U/mL of protease activity and 580 mg/mL of total amino acids. The produced peptone demonstrated a superior amino acid profile compared to commercial peptones, with a remarkably higher total amino acid content of 621.556 mg/g and elevated levels of essential amino acids like aspartic acid (37.745%), glutamic acid (90.876%), glycine (117.272%), and alanine (50.373%). Characterization revealed optimal pH and temperature conditions of around pH 8 and 50-60°C, respectively, for the proteolytic activity. The Michaelis-Menten and Lineweaver-Burk plots determined a Km of 0.5 mg/mL and Vmax of 174.08 U/mL suggesting cooperative substrate binding and providing insights into the enzyme's maximum rate and affinity. The produced peptone exhibited minimal cytotoxicity at lower concentrations (≤ 1 mg/mL), with cell viability exceeding 94% against normal human skin fibroblast (HSF) cells. However, higher concentrations (≥ 3 mg/mL) displayed increased cytotoxic effects. Moreover, the results strongly indicate that the produced peptone, particularly at 0.5% concentration, is an effective nitrogen source for B. subtilis cultivation, demonstrating its potential for biotechnological applications. This study successfully valorized poultry waste by developing a sustainable and cost-effective alternative to commercial peptones, contributing to waste valorization and sustainable biotechnological processes.

Dataset Information

Optimization of Cr⁶⁺ Removal by Bacillus subtilis Strain SZMC 6179J from Chromium-Containing Soil.

Publications

Optimization of Cr<sup>6+</sup> Removal by <i>Bacillus subtilis</i> Strain SZMC 6179J from Chromium-Containing Soil.

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