Pre-purification of diatom pigment protein complexes provides insight into the heterogeneity of FCP complexes.

ABSTRACT:

Background

Although our knowledge about diatom photosynthesis has made huge progress over the last years, many aspects about their photosynthetic apparatus are still enigmatic. According to published data, the spatial organization as well as the biochemical composition of diatom thylakoid membranes is significantly different from that of higher plants.

Results

In this study the pigment protein complexes of the diatom Thalassiosira pseudonana were isolated by anion exchange chromatography. A step gradient was used for the elution process, yielding five well-separated pigment protein fractions which were characterized in detail. The isolation of photosystem (PS) core complex fractions, which contained fucoxanthin chlorophyll proteins (FCPs), enabled the differentiation between

SUBMITTER: Kansy M 

PROVIDER: S-EPMC7539453 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature