Co-expression of human calreticulin significantly improves the production of HIV gp140 and other viral glycoproteins in plants.

ABSTRACT: Plant molecular farming (PMF) is rapidly gaining traction as a viable alternative to the currently accepted paradigm of producing biologics. While the platform is potentially cheaper and more scalable than conventional manufacturing systems, expression yields and appropriate post-translational modifications along the plant secretory pathway remain a challenge for certain proteins. Viral fusion glycoproteins in particular are often expressed at low yields in plants and, in some cases, may not be appropriately processed. Recently, however, transiently or stably engineering the host plant has shown promise as a strategy for producing heterologous proteins with more complex maturation requirements. In this study we investigated the co-expression of a suite of human chaperones to improve the pr

SUBMITTER: Margolin E 

PROVIDER: S-EPMC7540014 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature