CD47 antibody suppresses isoproterenol-induced cardiac hypertrophy through activation of autophagy.

ABSTRACT: In this study, we investigated whether CD47 antibody could attenuate isoproterenol (ISO)-induced cardiac hypertrophy in mice and H9C2 cells. Cardiac hypertrophy was induced by intraperitoneal (i.p.) injection of ISO (60 mg.kg^-1.d^-1 in 100 µl of sterile normal saline) daily for 14 days, and cell hypertrophy was induced by ISO (10^-5 mol/l) for 48 h. The injury was confirmed by increased levels of lactate dehydrogenase (LDH) and creatine kinase MB (CK-MB), increased heart-to-body weight (HW/BW) ratio and visible cardiac fibrosis. Apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Autophagic flux in H9c2 cells was monitored by TEM and mRFP-GFP-LC3 virus transfection. The expression levels of Cleave

SUBMITTER: Li Y 

PROVIDER: S-EPMC7540123 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature