Novel atherogenic pathways from the differential transcriptome analysis of diabetic epicardial adipose tissue.

ABSTRACT:

Background and aim

To evaluate the epicardial adipose tissue (EAT) transcriptome in comparison to subcutaneous fat (SAT) in coronary artery disease (CAD) and type 2 diabetes (T2DM).

Methods and results

SAT and EAT samples were obtained from subjects with T2DM and CAD (n = 5) and those without CAD with or without T2DM (=3) undergoing elective cardiac surgery. RNA-sequencing analysis was performed in both EAT and SAT. Gene enrichment analysis was conducted to identify pathways affected by the differentially expressed genes. Changes of top genes were verified by quantitative RT-PCR (qRT-PCR), western blot, and immunofluorescence. A total of 592 genes were differentially expressed in diabetic EAT, whereas there was no obvious changes in SAT transcriptome between diabetics and no

SUBMITTER: Camarena V 

PROVIDER: S-EPMC7540222 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature