Spectral Unmixing-Based Reaction Monitoring of Transformations between Nucleosides and Nucleobases.

ABSTRACT: The increased interest in (enzymatic) transformations between nucleosides and nucleobases has demanded the development of efficient analytical tools. In this report, we present an update and extension of our recently described method for monitoring these reactions by spectral unmixing. The presented method uses differences in the UV absorption spectra of nucleosides and nucleobases after alkaline quenching to derive their ratio based on spectral shape by fitting normalized reference spectra. It is applicable to a broad compound spectrum comprising more than 35 examples, offers HPLC-like accuracy, ease of handling and significant reductions in both cost and data acquisition time compared to other methods. This contribution details the principle of monitoring reactions by spectral unmixing,

SUBMITTER: Kaspar F 

PROVIDER: S-EPMC7540295 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature