Minimal deuterium isotope effects in quantitation of dimethyl-labeled complex proteomes analyzed with capillary zone electrophoresis/mass spectrometry.

ABSTRACT: Stable heavy-isotope labeling is commonly used in quantitative proteomics. Several common techniques incorporate deuterium (² H) as the heavy isotopic label using reductive amination with formaldehyde. Compared with alternatives, dimethyl labeling reagents are inexpensive and the labeling chemistry is simple and rapid. However, the substitution of hydrogen by deuterium can introduce subtle changes in peptides' polarities, leading to a shift in chromatographic retention times between deuterated and nondeuterated peptides that can lead to quantification deviations. Capillary zone electrophoresis has emerged as a complementary separation for ESI-MS-based proteomics, including targeted and quantitative approaches. The extent to which the deuterium isotope effect impacts CZE-based pr

SUBMITTER: Yan X 

PROVIDER: S-EPMC7540333 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature