Electroenzymatic Nitrogen Fixation Using a MoFe Protein System Immobilized in an Organic Redox Polymer.

ABSTRACT: We report an organic redox-polymer-based electroenzymatic nitrogen fixation system using a metal-free redox polymer, namely neutral-red-modified poly(glycidyl methacrylate-co-methylmethacrylate-co-poly(ethyleneglycol)methacrylate) with a low redox potential of -0.58 V vs. SCE. The stable and efficient electric wiring of nitrogenase within the redox polymer matrix enables mediated bioelectrocatalysis of N₃^- , NO₂^- and N₂ to NH₃ catalyzed by the MoFe protein via the polymer-bound redox moieties distributed in the polymer matrix in the absence of the Fe protein. Bulk bioelectrosynthetic experiments produced 209±30 nmol NH₃  nmol MoFe^-1  h^-1 from N₂ reduction. ¹⁵ N₂

SUBMITTER: Lee YS 

PROVIDER: S-EPMC7540466 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature