Catalytically Active Hollow Fiber Membranes with Enzyme-Embedded Metal-Organic Framework Coating.

ABSTRACT: Metal-organic frameworks (MOFs) are suitable enzyme immobilization matrices. Reported here is the in situ biomineralization of glucose oxidase (GOD) into MOF crystals (ZIF-8) by interfacial crystallization. This method is effective for the selective coating of porous polyethersulfone microfiltration hollow fibers on the shell side in a straightforward one-step process. MOF layers with a thickness of 8 μm were synthesized, and fluorescence microscopy and a colorimetric protein assay revealed the successful inclusion of GOD into the ZIF-8 layer with an enzyme concentration of 29±3 μg cm^-2 . Enzymatic activity tests revealed that 50 % of the enzyme activity is preserved. Continuous enzymatic reactions, by the permeation of β-d-glucose through the GOD@ZIF-8 membranes, showed a 50 %

SUBMITTER: Bell DJ 

PROVIDER: S-EPMC7540569 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature