Applicability and reproducibility of acute myeloid leukaemia stem cell assessment in a multi-centre setting.

ABSTRACT: Leukaemic stem cells (LSC) have been experimentally defined as the leukaemia-propagating population and are thought to be the cellular reservoir of relapse in acute myeloid leukaemia (AML). Therefore, LSC measurements are warranted to facilitate accurate risk stratification. Previously, we published the composition of a one-tube flow cytometric assay, characterised by the presence of 13 important membrane markers for LSC detection. Here we present the validation experiments of the assay in several large AML research centres, both in Europe and the United States. Variability within instruments and sample processing showed high correlations between different instruments (R_pearson  > 0·91, P < 0·001). Multi-centre testing introduced variation in reported LSC percentages but was fou

SUBMITTER: Hanekamp D 

PROVIDER: S-EPMC7540683 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature