Optimization in Detection of Antigen-Specific T Cells Through Differentially Labeled MHC Multimers.

ABSTRACT: A large variety of fluorescent molecules are used on a regular basis to tag major histocompatibility complex (MHC) multimers for detection of antigen-specific T cells. We have evaluated the way in which the choice of fluorescent label can impact the detection of MHC multimer binding T cells in an exploratory proficiency panel where detection of MHC multimer binding T cells was assessed across 16 different laboratories. We found that the staining index (SI) of the multimer reagent provided the best direct correlation with the value of a given fluorochrome for T cell detection studies. The SI is dependent on flow cytometer settings and chosen antibody panel; hence, the optimal fluorochrome selection may differ from lab to lab. Consequently, we describe a strategy to evaluate performance of t

SUBMITTER: Pedersen NW 

PROVIDER: S-EPMC7540688 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature