Immunosuppressive role of CD11b⁺ CD33⁺ HLA-DR^- myeloid-derived suppressor cells-like blast subpopulation in acute myeloid leukemia.

ABSTRACT:

Objective

Myeloid-derived suppressor cells (MDSCs) facilitate tumor growth and development by suppressing T cell function; however, their role in acute myeloid leukemia (AML) remains unclear. Here, we investigated the immunosuppressive role and prognostic value of blasts with an MDSC-like phenotype.

Methods

CD11b⁺ CD33⁺ HLA-DR^- MDSC-like blasts from bone marrow mononuclear cells of patients with AML were analyzed. To investigate their T cell-suppressing function, MDSC-like blasts were isolated using flow cytometry and co-cultured with CD8⁺ cytotoxic T cells and NB4 leukemic cells. Treatment outcomes were then compared between the MDSC-like blasts low (≤9.76%) and high (>9.76%) groups to identify clinical significance.

Results

SUBMITTER: Hyun SY 

PROVIDER: S-EPMC7541151 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature