Proteotranscriptomics assisted gene annotation and spatial proteomics of Bombyx mori BmN4 cell line.

ABSTRACT:

Background

The process of identifying all coding regions in a genome is crucial for any study at the level of molecular biology, ranging from single-gene cloning to genome-wide measurements using RNA-seq or mass spectrometry. While satisfactory annotation has been made feasible for well-studied model organisms through great efforts of big consortia, for most systems this kind of data is either absent or not adequately precise.

Results

Combining in-depth transcriptome sequencing and high resolution mass spectrometry, we here use proteotranscriptomics to improve gene annotation of protein-coding genes in the Bombyx mori cell line BmN4 which is an increasingly used tool for the analysis of piRNA biogenesis and function. Using this approach we provide the exact coding sequence a

SUBMITTER: Levin M 

PROVIDER: S-EPMC7541253 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature