Multiparameter flow cytometric detection and quantification of senescent cells in vitro.

ABSTRACT: It has been over half a century since cellular senescence was first noted and characterized, and yet no consensus senescent marker has been reliably established. This challenge is compounded by the complexity and heterogenic phenotypes of senescent cells. This necessitates the use of multiple biomarkers to confidently characterise senescent cells. Despite cytochemical staining of senescence associated-beta-galactosidase being a single marker approach, as well as being time and labour-intensive, it remains the most popular detection method. We have developed an alternative flow cytometry-based method that simultaneously quantifies multiple senescence markers at a single-cell resolution. In this study, we applied this assay to the quantification of both replicative and induced senescent prim

SUBMITTER: Adewoye AB 

PROVIDER: S-EPMC7541365 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature