Axonal Protection by Nicotinamide Riboside via SIRT1-Autophagy Pathway in TNF-Induced Optic Nerve Degeneration.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺) synthesis pathway has been involved in many biological functions. Nicotinamide riboside (NR) is widely used as an NAD⁺ precursor and known to increase NAD⁺ level in several tissues. The present study aimed to examine the effect of NR on tumor necrosis factor (TNF)-induced optic nerve degeneration and to investigate whether it alters SIRT1 expression and autophagic status in optic nerve. We also examined the localization of nicotinamide riboside kinase 1 (NRK1), which is a downstream enzyme for NR biosynthesis pathway in retina and optic nerve. Intravitreal injection of TNF or TNF plus NR was performed on rats. The p62 and LC3-II protein levels were examined to evaluate autophagic flux in optic nerve. Immunohistochemi

SUBMITTER: Kitaoka Y 

PROVIDER: S-EPMC7541376 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature