An untargeted metabolomics strategy to measure differences in metabolite uptake and excretion by mammalian cell lines.

ABSTRACT:

Introduction

It is widely but erroneously believed that drugs get into cells by passing through the phospholipid bilayer portion of the plasma and other membranes. Much evidence shows, however, that this is not the case, and that drugs cross biomembranes by hitchhiking on transporters for other natural molecules to which these drugs are structurally similar. Untargeted metabolomics can provide a method for determining the differential uptake of such metabolites.

Objectives

Blood serum contains many thousands of molecules and provides a convenient source of biologically relevant metabolites. Our objective was to detect and identify metabolites present in serum, but to also establish a method capable of measure their uptake and secretion by different cell lines.

Methods

SUBMITTER: Wright Muelas M 

PROVIDER: S-EPMC7541387 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature