Decreased Frequency of Intestinal CD39⁺ γδ⁺ T Cells With Tissue-Resident Memory Phenotype in Inflammatory Bowel Disease.

ABSTRACT: The ectoenzymes CD39 and CD73 play a major role in controlling tissue inflammation by regulating the balance between adenosine triphosphate (ATP) and adenosine. Still, little is known about the role of these two enzymes and ATP and its metabolites in the pathophysiology of inflammatory bowel disease (IBD). We isolated mononuclear cells from peripheral blood and lamina propria of the large intestine of patients diagnosed with IBD and of healthy volunteers. We then comprehensively analyzed the CD39 and CD73 expression patterns together with markers of activation (HLA-DR, CD38), differentiation (CCR7, CD45RA) and tissue-residency (CD69, CD103, CD49a) on CD4⁺, CD8⁺, γδ⁺ T cells and mucosa-associated invariant T cells using flow cytometry. CD39 expression levels

SUBMITTER: Libera J 

PROVIDER: S-EPMC7541837 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature