Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species.

ABSTRACT: Species identification of unknown biological samples is of fundamental importance for forensic applications, especially in crime detection, poaching, and illegal trade of endangered animals as well as meat fraud. In this study, a novel panel was developed to simultaneously identify 10 different animal species (Gallus domesticus, Anas platyrhynchos domesticus, Ovis aries, Sus scrofa domesticus, Bos taurus, Equus caballus, Columba livia domestica, Rattus norvegicus, Mus musculus, and Canis lupus familiaris) and human beings by amplifying 22 short tandem repeat (STR) loci in a multiplex PCR using a set of five fluorescently labeled dyes. This novel 22-STR panel was validated by optimization of PCR conditions as well as spec

SUBMITTER: Cui W 

PROVIDER: S-EPMC7541953 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature