NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression.

ABSTRACT:

Background

Nuclear factor of activated T cells 2 (NFAT2) has been reported to regulate the development and malignancy of few tumors. In this study, we aimed to explore the effect of NFAT2 expression on cell fate of HepG2 cell and its potential mechanisms.

Methods

Firstly, the pcDNA3.1-NFAT2 plasmid was transfected into HepG2 cells to construct NFAT2 overexpressed HepG2 cells. Then, the chemical count kit-8 cell viability assay, Annexin V-FITC apoptosis detection, EdU labeling proliferation detection, transwell and wound healing experiments were performed. The expression of Egr2 and FasL, and the phosphorylation of AKT and ERK, after ionomycin and PMA co-stimulation, was detected, while the Ca²⁺ mobilization stimulated by K⁺ solution was determined. At l

SUBMITTER: Wang J 

PROVIDER: S-EPMC7542386 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature