Molecular Detection and Typing of Pathogenic <i>Leptospira</i> in Febrile Patients and Phylogenetic Comparison with <i>Leptospira</i> Detected among Animals in Tanzania.

ABSTRACT: Molecular data are required to improve our understanding of the epidemiology of leptospirosis in Africa and to identify sources of human infection. We applied molecular methods to identify the infecting Leptospira species and genotypes among patients hospitalized with fever in Tanzania and compared these with Leptospira genotypes detected among animals in Tanzania to infer potential sources of human infection. We performed lipL32 real-time PCR to detect the presence of pathogenic Leptospira in acute-phase plasma, serum, and urine samples obtained from study participants with serologically confirmed leptospirosis and participants who had died with febrile illness. Leptospira blood culture was also performed. In positive specimens, we performed species-spec

SUBMITTER: Allan KJ 

PROVIDER: S-EPMC7543812 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature