Pannexin 1 Regulates Dendritic Protrusion Dynamics in Immature Cortical Neurons.

ABSTRACT: The integration of neurons into networks relies on the formation of dendritic spines. These specialized structures arise from dynamic filopodia-like dendritic protrusions. It was recently reported that cortical neurons lacking the channel protein pannexin 1 (PANX1) exhibited higher dendritic spine densities. Here, we expanded on those findings to investigate, at an earlier developmental time point (with more abundant dendritic protrusions), whether differences in the properties of dendritic protrusion dynamics could contribute to this previously discovered phenomenon. Using a fluorescent membrane tag (mCherry-CD9-10) to visualize dendritic protrusions in developing neurons [at 10 d in vitro (DIV10)], we confirmed that lack of PANX1 led to higher protrusion density, while transient transfec

SUBMITTER: Sanchez-Arias JC 

PROVIDER: S-EPMC7544189 | biostudies-literature | 2020 Jul/Aug

REPOSITORIES: biostudies-literature