Rapid, robust plasmid verification by de novo assembly of short sequencing reads.

ABSTRACT: Plasmids are a foundational tool for basic and applied research across all subfields of biology. Increasingly, researchers in synthetic biology are relying on and developing massive libraries of plasmids as vectors for directed evolution, combinatorial gene circuit tests, and for CRISPR multiplexing. Verification of plasmid sequences following synthesis is a crucial quality control step that creates a bottleneck in plasmid fabrication workflows. Crucially, researchers often elect to forego the cumbersome verification step, potentially leading to reproducibility and-depending on the application-security issues. In order to facilitate plasmid verification to improve the quality and reproducibility of life science research, we developed a fast, simple, and open source pipeline for assembly an

SUBMITTER: Gallegos JE 

PROVIDER: S-EPMC7544192 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature