MiR-125a-5p regulates megakaryocyte proplatelet formation via the actin-bundling protein L-plastin.

ABSTRACT: There is heritability to interindividual variation in platelet count, and better understanding of the regulating genetic factors may provide insights for thrombopoiesis. MicroRNAs (miRs) regulate gene expression in health and disease, and megakaryocytes (MKs) deficient in miRs have lower platelet counts, but information about the role of miRs in normal human MK and platelet production is limited. Using genome-wide miR profiling, we observed strong correlations among human bone marrow MKs, platelets, and differentiating cord blood-derived MK cultures, and identified MK miR-125a-5p as associated with human platelet number but not leukocyte or hemoglobin levels. Overexpression and knockdown studies showed that miR-125a-5p positively regulated human MK proplatelet (PP) formation in vitro. Inhi

SUBMITTER: Bhatlekar S 

PROVIDER: S-EPMC7544541 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature